Journal: Current protocols in cytometry
Article Title: CyTOF Measurement of Immunocompetence across Major Immune Cell Types
doi: 10.1002/cpcy.27
Figure Lengend Snippet: Example of a Staining Panel
Article Snippet: Here we use cryopreserved PBMC, although this protocol is also compatible with fresh human PBMC. table ft1 table-wrap mode="anchored" t5 caption a7 Metal label Specificity Antibody Clone Source 140Ce Beads - Fluidigm 141Pr CD25 MA251, BD in-house 142Nd CD19 HIB19 Fluidigm 143Nd IL-10 JES3-9D7, Biolegend in-house 144Nd IL-4 MP4-25D2 Fluidigm 145Nd CD4 RPA-T4 Fluidigm 146Nd CD8 RPA-T8 Fluidigm 147Sm CD20 2H7 Fluidigm 148Nd CD57 HCD57, Biolegend in-house 149Sm CTLA-4 14D3, eBioscience in-house 150Nd MIP1β D21-1351 Fluidigm 151Eu CD107a H4A3 Fluidigm 152Sm TNFα Mab11 Fluidigm 153Eu CD45RA HI100 Fluidigm 154Sm CD3 UCHT1 Fluidigm 155Gd CD28 L283, BD in-house 156Gd CD38 HB-7, BD in-house 157Gd HLA-DR G46-6, BD in-house 158Gd CD33 WM53 Fluidigm 159Tb GMCSF BVD2-21C11 Fluidigm 160Gd CD14 M5E2 Fluidigm 161Dy IFNγ 4S.B3, eBioscience in-house 162Dy CD69 MCA 1442 Fluidigm 163Dy TCRγδ B1, Biolegend in-house 164Dy IL-17 N49-853 Fluidigm 165Ho CD127 A019D5 Fluidigm 166Er IL-2 MQ1-17h12 Fluidigm 167Er CD27 L128 Fluidigm 168Er CD154 (CD40L) 24-31 Fluidigm 169Tm CCR7 150503, R&D Systems in-house 170Er PD1 EH12.1, BD in-house 171Yb Granzyme B GB11 Fluidigm 172Yb PD-L2 24F.10C12 Fluidigm 173Yb Perforin B-D48, Abcam in-house 174Yb CD16 3G8, Biolegend in-house 175Lu PD-L1 29E.2A3 in-house 176Yb CD56 NCAM16.2 DVS Open in a separate window Example of a Staining Panel First, we begin with thawing and resting of PBMC, which is followed by stimulation ( ).
Techniques: Staining
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